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By synthesizing a molecule that could permeate the cell membrane and selectively inhibit NNMT, researchers discovered they could fundamentally alter the metabolic profile of the cell

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Ersoy S, Kesiktas FN, Sirin B, Bugdayci D, Paker N

To circumvent this difficulty and to reduce the possibility of suppressor mutations that compensate for chronic loss of V-ATPase structural or assembly genes, we fused an AID* degron domain (a 71-114 truncated fragment of the IAA17 protein that proved to work efficiently in budding yeast) 24 to the endogenous Vma2 protein

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